About The Medical Condition Nested Polymerase Chain Reaction

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Find the definition of the medical term Nested Polymerase Chain Reaction. Nested Polymerase Chain Reaction defined and explained for easy understanding at the Medical Conditions from ClusterMed.info.

Nested Polymerase Chain Reaction

Nested Polymerase Chain Reaction Medical Condition Defined & Explained
Nested Polymerase Chain Reaction Medical Condition

What's The Definition Of The Medical Condition Nested Polymerase Chain Reaction?

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

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