About The Medical Condition PCR, Inverse


Find the definition of the medical term PCR, Inverse. PCR, Inverse defined and explained for easy understanding at the Medical Conditions from ClusterMed.info.

PCR, Inverse

PCR, Inverse Medical Condition Defined & Explained
PCR, Inverse Medical Condition

What's The Definition Of The Medical Condition PCR, Inverse?

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

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