About The Medical Condition Reaction, Ligase Chain
Find the definition of the medical term Reaction, Ligase Chain. Reaction, Ligase Chain defined and explained for easy understanding at the Medical Conditions from ClusterMed.info.
Reaction, Ligase Chain
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What's The Definition Of The Medical Condition Reaction, Ligase Chain?
A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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