About The Medical Condition Reactions, Polymerase Chain
Find the definition of the medical term Reactions, Polymerase Chain. Reactions, Polymerase Chain defined and explained for easy understanding at the Medical Conditions from ClusterMed.info.
Reactions, Polymerase Chain
Reactions, Polymerase Chain Medical Condition |
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What's The Definition Of The Medical Condition Reactions, Polymerase Chain?
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
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